Publications

M. Papalian, E. Lafer, R. Wong and B.D. Stollar. Reaction of Systemic Lupus Erythematosus Antinative DNA Antibodies with Native DNA Fragments from 20-1200 Base Pairs. J. Clin. Invest. 65, 469-477 (1980).

C.A. Andrzejewski, Jr., J. Rauch, E. Lafer, B.D. Stollar and R. Schwartz. Antigen Binding Diversity and Idiotypic Cross-Reactions Among Hybridoma Autoantibodies to DNA. J. of Immunol. 126, 226-231 (1981).

R. Kisliuk, Y. Gaumont, E. Lafer, C. Baugh and J. Montgomery. Polyglutamyl Derivatives of Tetrahydrofolate as Substrates for Lactobacillus casei Thymidylate Synthase. Biochemistry 20, 929-934 (1981).

E.M. Lafer, J. Rauch, C.A. Andrzejewski, Jr., D. Mudd, B.C. Furie, B. Furie, R.S. Schwartz and B.D. Stollar. Polyspecific Monoclonal Lupus Autoantibodies Reactive with Both Polynucleotides and Phospholipids. J. Exp. Med. 153, 897-907 (1981).

E.M. Lafer, A. Moller, A. Nordheim, B. D. Stollar and A. Rich. Antibodies Specific for Left-Handed Z-DNA. Proc. Nat. Acad. Sci. 78, 3546-3550 (1981).

A. Nordheim, M.L. Pardue, E.M. Lafer, A. Moller, B.D. Stollar and A. Rich. Antibodies to Left-Handed Z-DNA Bind to Interband Regions of Drosophila Polytene Chromosomes. Nature 294, 417-422 (1981).

A. Moller, J.E. Gabriels, E.M. Lafer, A. Nordheim, A. Rich and B.D. Stollar. Monoclonal Antibodies Recognize Different Parts of Z-DNA. J. Biol. Chem. 257, 12081-12085 (1982).

A. Nordheim, E.M. Lafer, L.J. Peck, J.C. Wang, B.D. Stollar and A. Rich. Negatively Supercoiled Plasmids Contain Left-handed Z-DNA Segments As Detected by Specific Antibody Binding. Cell 31, 309-318 (1982).

E.M. Lafer, R.P.C. Valle, A. Moller, A. Nordheim, P.S. Schur, A. Rich and B.D. Stollar. Z-DNA Specific Antibodies in Human Systemic Lupus Erythematosus. J. Clin. Invest. 71, 314-321 (1983).

H.J. Lipps, A. Nordheim, E.M. Lafer, D. Ammermann, B.D. Stollar and A. Rich. Antibodies Against Z-DNA React With the Macronucleus but not the Micronucleus of the Hypotrichous Ciliate Stylonichia Mytilus. Cell 32, 435-441 (1983).

E. Lafer, A. Moller, R.P.C. Valle, A. Nordheim, A. Rich and B.D. Stollar. Antibody Recognition of Z-DNA. Cold Spring Harbor Symp. Quant. Biol. 47, 155-162 (1983).

M. Tomasz, J.K. Barton, C.C. Magliozzo, D. Tucker, E.M. Lafer and B.D. Stollar. Lack of Z-DNA Conformation in Mitomycin-Modified Polynucleotides Having Inverted Circular Dichroism. Proc. Natl. Acad. Sci. USA 80, 2874-2878 (1983).

E.M. Lafer and B.D. Stollar. The Specificity of Antibodies Elicited by Poly(dG).Poly(dC). J. Biomolecular Structure and Dynamics 2, 487-494 (1984).

B.D. Stollar, E.M. Lafer, A. Nordheim, M.L. Pardue and A. Rich. Immunological Probes for the Biological Significance of Z-DNA. In Progress in Clinical and Biological Research 172b, The Molecular Basis of Cancer, R. Rein, ed., Alan R. Liss, Inc., 323-332 (1985).

E.M. Lafer, R. Sousa, B. Rosen, A. Hsu and A. Rich. Isolation and Characterization of Z-DNA Binding Proteins From Wheat Germ. Biochemistry 24, 5070-5076 (1985).

E.M. Lafer, R. Sousa and A. Rich. Anti-Z-DNA Antibody Binding Can Stabilize Z-DNA in Relaxed and Linear Plasmids Under Physiological Conditions. EMBO Journal 13B, 3655-3660 (1985).

E.M. Lafer, R. Sousa, R. Ali, A. Rich and B.D. Stollar. The Effect of Anti-Z-DNA Antibodies on the B-DNA - Z-DNA Equilibrium. J. Biol. Chem. 261, 6438-6443 (1986).

E.M. Lafer, R. Sousa, and A. Rich. E. coli Z-DNA Binding Proteins: Purification, Monoclonal Antibody Generation, and Gene Isolation. J. Mol. Biol., 203, 511-516 (1988).

R. Sousa, N. Hrinya Tannery, and E.M. Lafer. In situ Hybridization Mapping of Glucocorticoid Receptor mRNA in Rat Brain. Molecular Endocrinology 3, 481-494 (1989).

R. Sousa, J.P. Rose, Y.J. Chung, E.M. Lafer and B.C. Wang. Single Crystals of T7 RNA Polymerase. PROTEINS: Structure, Function, and Genetics 5, 266-270 (1989).

R. Sousa, N. Hrinya Tannery, and E.M. Lafer. Two New Monoclonal Antibodies Provide Immunohistochemical Evidence for the Unique Biochemical Similarity of the Mouse Globus Pallidus, Entopeduncular Nucleus, and Substantia Nigra pars Reticulata. Neuroscience 34, 403-410 (1990).

Y.J. Chung, R.J. Sousa, J.P. Rose, E.M. Lafer, and B.C. Wang. Crystallographic Structure of Phage T7 RNA Polymerase at a Resolution of 4 Å. In Structure and Function of Nucleic Acids and Proteins, F. Wu and C.W. Wu, eds, pp. 55-59, Raven Press, N.Y (1990).

Y.J. Chung, R.J. Sousa, J.P. Rose, E.M. Lafer, and B.C. Wang. Three-dimensional Structure of Bacteriophage T7 RNA Polymerase. In Advances in Applied Biotechnology Series, Vol. 7: Gene Regulation and AIDS: Transcriptional Activation, Retroviruses, and Pathogenesis, T.S. Papas, ed., Gulf Publishing Co., Houston, pp. 3-7 (1990).

R. Sousa, Y.J. Chung, W.T. McCallister, B.C. Wang, and E.M. Lafer. Single Crystals of a Chimeric T7/T3 RNA Polymerase with T3 Promoter Specificity and a Non-Processive T7 RNAP Mutant. J. Biol. Chem. 265, 21430-21432 (1990).

R. Sousa, E.M. Lafer, and B.C. Wang. Preparation of Crystals of T7 RNA Polymerase Suitable For High Resolution X-Ray Structure Analysis. J. Crystal Growth 110, 237-246 (1991)

R. Sousa, Y.J. Chung, B.C. Wang, and E.M. Lafer. Preparation of Crystals of a Chimeric T7/T3 RNA Polymerase with T3 Promoter Specificity. J. Crystal Growth 122, 366-374 (1992).

D. Patra, E.M. Lafer, and R. Sousa. Isolation and Characterization of Mutant Bacteriophage T7 RNA Polymerases, J. Mol. Biol. 224, 307-318 (1992).

R. Sousa, D. Patra, and E.M. Lafer. Model for the Mechanism of Bactreiophage T7 RNAP Transcription Initiation and Termination. J. Mol. Biol. 224, 319-334 (1992).

R. Sousa, N. Hrinya Tannery, S. Zhou, and E.M. Lafer. Characterization of a Novel Synapse-Specific Protein: I. Developmental Expression and Cellular Localization of the F1-20 Protein and mRNA, J. Neurosci. 12, 2130-2143 (1992).

S. Zhou, R. Sousa, N. Hrinya Tannery, and E.M. Lafer. Characterization of a Novel Synapse- Specific Protein: II. cDNA Cloning and Sequence Analysis of the F1-20 Protein, J. Neurosci. 12, 2144-2155 (1992).

G. Bonner, D. Patra, E.M. Lafer, and R. Sousa. Mutations in T7 RNA Polymerase that Support the Proposal for a Common Polymerase Active Site Structure. EMBO J. 11, 3767-3775 (1992).

S. Zhou, N. Hrinya Tannery, J. Yang, S. Puszkin, and E.M. Lafer. The Synapse-Specific Phospho-protein F1-20 is Identical to the Clathrin Assembly Protein AP-3. J. Biol. Chem., 268, 12655-12662 (1993).

G. Bonner, E.M. Lafer and R.Sousa. Characterization of a Set of T7 RNAP Active Site Mutants, J. Biol. Chem. 269, 25120-25128(1994).

G. Bonner, E.M. Lafer and R. Sousa. The Thumb Subdomain of T7 RNA Polymerase Functions to Stabilize the Ternary Complex During Processive Transcription, J. Biol. Chem. 269, 25129-25136(1994).

W. Ye, N. Ali, M. Bembenek, S.B. Shears and E.M. Lafer. Inhibition of Clathrin Assembly by High-Affinity Binding of Specific Inositol Polyphosphates to the Synapse-Specific Clathrin Assembly Protein AP-3. J. Biol. Chem. 270: 1564-1568 (1995).

W. Ye and E.M. Lafer. Bacterially Expressed F1-20/AP-3 Assembles Clathrin Into Cages With a Narrow Size Distribution: Implications for the Regulation of Quantal Size During Neurotransmission. J. Neurosci. Res. 41: 15-26 (1995).

W. Ye and E.M. Lafer. Clathrin Binding and Assembly Activities of Expressed Domains of the Synapse-Specific Clathrin Assembly Protein AP-3. J. Biol. Chem. 270: 10933-10939 (1995)

W. Hao, Z. Tan, K. Prasad, K.K. Reddy, J. Chen, G.D. Prestwich, J.R. Falck, S.B. Shears, and E.M. Lafer. Regulation of AP-3 Function by Inositides: Identification of Phosphatidylinositol 3,4,5-trisphosphate as a Potent Ligand. J. Biol. Chem. 272: 6393-6398 (1997).

A.A. Profit, J. Chen, Q.-M. Gu, A. Chaudhary, K. Prasad, E.M. Lafer and G.D. Prestwich. Probing the Phosphoinositide Binding Site of the Clathrin Assembly Protein AP-2 with Photoaffinity Labels. Archives Biochem. Biophys. 357: 85-94 (1998).

W. Hao, Z. Luo, L. Zheng, K. Prasad, and E.M. Lafer. AP180 and AP-2 Interact Directly in a Complex that Cooperatively Assembles Clatrhin. J. Biol. Chem. 274: 22785-22794 (1999).

J.R. Morgan, X. Xiao, M. Womack, K. Prasad, G.J. Augustine, E.M. Lafer. A Role for the Clathrin Assembly Domain of AP180 in Synaptic Vesicle Endocytosis. J. Neurosci. 19: 10201-10212 (1999).

J.R. Morgan, K. Prasad, W. Hao, G.J. Augustine, and E.M. Lafer. A Conserved Clathrin Assembly Motif is Essential for Synaptic Vesicle Endocytosis. J. Neurosci. 20: 8667-8676 (2000).

J.R. Morgan, E.M. Lafer, and G.J. Augustine. Rapid-Fire Synaptic Vesicle Exocytosis. Neuron 30: 5-7 (2001).

J.R. Morgan, K. Prasad, S. Jin, and G.J. Augustine, and E.M. Lafer. Uncoating of Clathrin-Coated Vesicles in Presynaptic Terminals: Roles for Hsc70 and Auxilin. Neuron 32: 289-300 (2001).

L. Carvelli, J.A. Morón, K.M. Kahlig, J.V. Ferrer, N. Sen, J.D. Lechleiter, L.M.F. Leeb-Lundberg, G. Merrill, E.M. Lafer, L.M. Ballou, T.S. Shippenberg, J.A. Javitch, R.Z. Lin, and A. Galli. PI 3-Kinase Regulation of Dopamine Uptake. J. Neurochem. 81: 859-869 (2002).

E.M. Lafer. Clathrin-Protein Interactions. Traffic 3: 513-520 (2002).

J.R. Morgan, G.J. Augustine, E.M. Lafer. Synaptic Vesicle Endocytosis: The Races, Places, and Molecular Faces. NeuroMolecular Medicine 2: 101-114 (2002).

J. Jiang, A.B. Taylor, K. Prasad, Y. Ishikawa-Brush, P.J. Hart, E.M. Lafer, and R. Sousa. Structure-Function Analysis of the Auxilin J-Domain Reveals an Extended Hsc70 Interaction Interface. Biochemistry 42: 5748-5753 (2003).

J.R. Morgan, K. Prasad, S. Jin, G.J. Augustine, and E.M. Lafer. Eps15 Homology Domain â NPF Motif Interactions Regulates Clathrin Coat Assembly During Synaptic Vesicle Recycling. J. Biol. Chem. 278: 33583-92 (2003).

A. Morón, I. Zakharova, J.V. Ferrer, G.A. Merrill, B. Hope, E.M. Lafer, Z.C. Lin, J.B. Wang, J.A. Javitch, A. Galli, and T.S. Shippenberg. Mitogen-Activated Protein Kinase Regulates Dopamine Transporter Surface Expression and Dopamine Transport Capacity. J. Neurosci. 23:8480-8 (2003).

P.S.N. Rowe, I.R. Garrett, P.M. Schwarz, D.L. Carnes, E.M. Lafer, G.R. Mundy, and G.E. Gutierrez. Surface Plasmon Resonance (SPR) confirms that MEPE binds to PHEX via the MEPE-ASARM-motif: A model for impaired mineralization in X-linked rickets (HYP). Bone 36:33-46 (2005).

S.Yu, X. Yin, E.M. Lafer and J.X. Jiang. Developmental Regulation of the Direct Interaction between the Intracellular Loop of Connexin 45.6 and the C-terminus of Major Intrinsic Protein (Aquaporin-0). J. Biol. Chem. 280:22081-90 (2005).

J. Jiang, K. Prasad, E.M. Lafer and R. Sousa. Structural Basis of Interdomain Communication in the Hsc70 Chaperone. Molecular Cell 20:513-524 (2005).

G.J. Augustine, J.R. Morgan, C. Villalba-Galea, S. Jin, K. Prasad, and E.M. Lafer. Clathrin and Synaptic Vesicle Endocytosis: Studies at the squid giant synapse. Biochemical Society Transactions, in press (2006).

A.J. Jin, K. Prasad, P.D. Smith, E.M. Lafer and R.J. Nossal. Measuring the elasticity of clathrin coated vesicles via atomic force microscopy. Biophysical Journal, in press (2006).

J. Jiang, E.M. Lafer, and R. Sousa. Crystallization of a Functionally Intact Bovine Hsc70.  ACTA F, in press (2006)

M. Ferguson, K. Prasad, D.L. Sackett, H. Boukari, E.M. Lafer and R. Nossal. The Conformation of Clathrin Triskelia in Solution. Biochemistry, submitted (2006).