Enterobacteria phage lambda
This page has working notes for SCH. It is not intended as a finished
presentation for public consumption.
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NC_001416
NRef
entries:
9626244.. 9626310, and
19263394..19263397
9626248 found in /home/user/hardies/families/sfi21clip/c1pb2.a2m
9626292 found in /home/user/hardies/families/sfi21hp170/hp170b1.a2m
(CI repressor)
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NP_040580.1;
9626244; nu1 (DNA packaging;181); DNA packaging protein
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Background: Small terminase
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Prodom has first 100 residues in a 7 member family: PD556941
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The rest is in PD013303 10 members with a structure: 107.. EVV..
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16 members on first round; includes N15 and prophages. 29 members in b1,
includes VHML.
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619 in c1; 604 in c3; at this point the 2nd domain drops out.
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785 in d1; 660 in d3;
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Named phage members are: lambda nu1; CP-933X,R; QSR', Qin, Gifsy-1, N15
gp1, VHML orf21, [Gifsy-2; Mx8 excisionase Uoi]; Last two found after MerR
poured in, so may be out of specific family.
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Other named genes are: MerR trans regulator; mer1; hmrR; cueR; PbrR; CadR;
Zn trans regulator; EncE; heavy metal-dependent trans. regulator; tipA;
Multidrug-efflux transporter 2 regulator; HmrR; NODULATION PROTEIN NOLA;
SoxR; GdnT; GlnR; SkgA; HspR; First ORF in transposon ISC1904;
Modification methylase Eco47II;
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study; The expanding model turned from small terminase-specific
to general helix turn helix model for the N terminal segment between rounds
b and c.
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Start with lam_nu1C (107..end).
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Check on lam_nu1Ca started on node5 7/24/03; 9 members, named phages in
a1: lambda, QSR', Qin=QIN, N15, CP-933R, Qin=21, Gifsy-1
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Tuneup needs killends -l, or else the model starts creeping into the
N terminal.
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lam_Nu1C in lam_nu1Ca2 is ungapped.
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The C terminal alone converged to 9 members by b1.
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Make model of lam_nu1Ca3 and screen lam_nu1b3, c3, d3.
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Finds: Ec-g15830359; CP-933R=Qin, phage 21, QSR', CP-933X, lambda, Ec-g15831434,Gifsy-1
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933X was eliminated from the C ter family because it was identical to another
entry. So nothing new hiding in lam_nu1b3..d3.
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Did lam_nu1C model vs smter and vice versa.- they don't seem to be relatable.
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NP_040581.1;
9626245; A (DNA packaging;641); DNA packaging protein
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50 members on first pass
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Accounted for in the global large terminase pfo family.
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leave behind
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NP_040582.1;
9626246; W (head-tail joining;68); head-tail joining protein
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PF02831, there is a structure (1hyw). Present in capsid and required
for addition of gpFii, and hence tail str. They prominantly have phage
21 gpH in their alignment.
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8 members on first round; 9 in b1 (added 933R).
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stalled at 9 in c1: Named phages and prophages =lambda,N15,Gifsy-1,CP-933O=CP-933R,
phage 21.
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NP_040583.1;
9626247; B (capsid component;533); capsid component
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portal protein
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48 members first round; phages were N15 and WO. 59 members in b1, including
Fels-1, Gifsy-1,2, wKue, phage 21, VHML, .
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58 in c1; 56 in c3;
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converged to 56 in d1; named phages and prophages = 12: lambda,CP-933X,CP-933R,CP-933O,N15,Gifsy-1,Fels-1,VHML,Gifsy-2,CP-933K,WO,CP-933U
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tune d
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NP_040584.1;
9626248; C (capsid component;439); capsid component
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C1p protease; accounted for in general clp family.
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leave behind
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NP_040585.1;
9626249; nu3 (capsid assembly;201); capsid assembly
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57 members after round 1.
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Numbers of them are proteases; 255 members in b1. Includes signal peptide
peptidases.
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Multiple members in some phages; cross homology with lambda C, above.
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Named: lambda,CP-933X,N15,CP-933R,WO,Gifsy-1,Felix01,VHML,phiE125
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leave behind
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NP_040586.1;
9626250; D (head-DNA stabilization;110); head-DNA stabilization
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13 members after round 1; stalled at b1; Named: lambda,CP-933O=CP-933R,CP-933X,N15,Gifsy-1
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stalled
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NP_040587.1;
9626251; E (capsid component;341); capsid component
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Major head protein
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10 members after round 1; stalled at b1, includes phi-80; named: lambda,CP-933X,N15,phi-80,Gifsy-1
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stalled.
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NP_040588.1;
9626252; Fi (DNA packaging;117); DNA packaging
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9 members after 1st round.; stalled on b1; named: lambda,CP-933X,Gifsy-1,N15
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stalled.
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NP_040589.1;
9626253; Fii (head-tail joining;117); head-tail joining
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11 members after 1st round; stalled on b1; named: lambda,CP-933X,N15,Gifsy-1
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stalled.
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NP_040590.1;
9626254; Z (tail component;192); tail component
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15 members a3; up to 16 on b1 (gifsy-2 added). Named: lambda,CP-933K,CP-933U,Fels-1,Gifsy-1,N15,Gifsy-2
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stalled at 16 in c1.
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NP_040591.1;
9626255; U (tail component;131); tail component
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13 members a3; stalled at 13 in b1; named: lambda=CP-933X,CP-933K,Gifsy-1,Fels-1,N15,Gifsy-2,CP-933U
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stalled.
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NP_040592.1;
9626256; V (tail component;256); tail component
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major tail protein
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111 members in a3, but lots of adhesion proteins and IgG domains.;
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Named in a3: lambda,CP-933X,CP-933U,CP-933K,Fels-1,N15,Gifsy-1,Gifsy-2,CP-933R,CP-933O,phiYeO3-12
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lam_V short 1..124 raises 15 members.
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Named in lam_Vshorta3: lambda,CP-933U,CP-933X,CP-933K,Gifsy-1,Fels-1,Gifsy-2,N15,CP-933R,CP-933O
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special case
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NP_040593.1;
9626257; G (tail component;140); tail component
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13 members in a3; up to 14 in b1; named in c3: lambda,CP-933X,CP-933O=CP-933R,CP-933U,N15,CP-933K,Gifsy-2,Gifsy-1
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stalled at 14 in c1.
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NP_040594.1;
9626258; T (tail component;144); tail component
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13 members in a3; up to 16 in b1; named: lambda=CP-933X,CP-933O=CP-933R,Gifsy-1,N15,Gifsy-2,CP-933U,Fels-1
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stalled at 16 in c1.
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NP_040595.1;
9626259; H (tail component;853); tail component
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tape measure protein.
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47 members in a3; picked up phiE125, D3, HK022, but had a human contaminant.
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Named: lambda,CP-933O,CP-933U,N15,Gifsy-1,CP-933K,Gifsy-2,Fels-1,LambdaSo,CP-933R,CP-933P,
HK97,CP-933V,phiE125,HK022,D3
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if sfi21btailfim is pursued, it should join with this.
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480 in b1: still named is lambda,CP-933O,CP-933U,N15,Gifsy-1,CP-933K,Gifsy-2,Fels-1,LambdaSo,CP-933R,CP-933P,
HK97,CP-933V,phiE125,HK022,D3
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special case.
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NP_040596.1;
9626260; M (tail component;109); tail component
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26 members in a3; 27 in b1; includes HK022, HK97, phiE125; but increase
is of a human contaminant A13
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Named: lambda,CP-933K,CP-933R,CP-933O=CP-933U,Gifsy-2,Gifsy-1,Fels-1,HK022,HK97,
LambdaSo,phiE125,N15
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study contamination problem.
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NP_040597.1;
9626261; L (tail component;232); tail component
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34 members in a3. up to 36 in b1, but increase from eucaryotic A13 component??
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Named in b1: lambda, CP-933O, CP-933K, CP-933N, CP-933U, CP-933V, CP-933P,
CP-933R, Gifsy-1, Fels-1, Gifsy-2, HK022, N15, HK97, LambdaSo, phiE125,
2120, CP-933M, CP-933X
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study A13 contamination problem.
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NP_040598.1;
9626262; K (tail component;199); tail component
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Background:
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199AA protein in tail module. As of Lambda II, there was no commitment
to whether it was incorporated into the mature tail, although clearly if
so there isn't much. Mutants in lam_K fail to assemble tails.
It is thought to be required for for assembly of the 25S tail "initiator"
complex (together with J, I, L, H, G, and M). Subsequently, V, U,
and Z are added. Subsequently it is variously called a tail assembly
protein or tail component protein, or just tail protein.
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It assembles into families with proteins called lysins, and hydrolases.
As such, it only makes sense if it is included within the tail. Most
of the protein, except the N terminal domain is included in the NLP/P60
family (pfam00877). Documentation on this familiy indicates that
some members are N-acetylmuramoyl-L-alanine amidases, whereas others may
be just peptidoglycan adhesion proteins. Either function makes sense
for inclusion in a tail fiber. Of the two papers recently analyzing
this family given below, Rigden is insistant that the motif as far as it
goes in lambda K is sufficient for hydrolase activity. They call
it a CHAP domain, (after a required C and H for catalysis).
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Rigden DJ, Jedrzejas MJ, Galperin MY. 2003. Amidase domains from bacterial
and phage autolysins define a family of gamma-D,L-glutamate-specific amidohydrolases
TRENDS IN BIOCHEMICAL SCIENCES 28 (5): 230-234 MAY 2003
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Anantharaman V, Aravind L. 2003. Evolutionary history, structural features
and biochemical diversity of the NlpC/P60 superfamily of enzymes GENOME
BIOLOGY 4 (2): art. no. R11 2003
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NLP/P60 family is pfam00877 with 227 members. hmmscore with lam_Kd3fw0_7
matched this family.
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Viral members:
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Q9FZU5 Q9FZU5 ORF 2. ORF 2. [251 residues] VirusesNeisseria meningitidis
phage 2120(1)
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Lactobacillus bacteriophage phi adh(1)
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Bacteriophage lambda(1)
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Bacteriophage phiE125(1)
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Bacteriophage N15(1)
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Bacteriophage HK022(1)
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Bacteriophage HK97(1)
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Structural representative = 1e0g
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Pfam00877 refers to associated domain IPR002482, which has 493 members
and is called LysM. IPR002482 documentation refers back to
PF01476 with 662 members.
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LysM structural representative: 1e01;
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Search of LysM vs. pfam00877 hmm indicates that this is a completely different
family; so "associated" must mean "next to".
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Bacteriophage members of LysM: hmmscore of this family with lam_Kd3fw0_7.mod
did not match
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Q9FZS8 [Bacteriophage phiam2] endolysin
[429 residues]
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Q9ZXD7 [Bacteriophage phi-105] orf46 [300
residues]
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Q38614 [Lactococcus lactis bacteriophage
tuc2009] lysin [428 residues]
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Q8LTP4 [Lactococcus lactis bacteriophage
ul36] putative lysin [429 residues]
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Q9AZ52 [Lactococcus lactis bacteriophage
tp901-1] lys [429 residues]
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Q9T1Z3 [Bacteriophage tpw22] lysin [429 residues]
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O03950 [Bacteriophage phig1e] lysin [442
residues]
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Q94ML9 [Bacillus amyloliquefaciens phage
morita2001] endolysin [258 residues]
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LYCV_BPB03 [Bacteriophage b103] lysozyme (ec 3.2.1.17) (lysis protein)
(muramidase) (endolysin)(morphogenesis protein 2) [263 residues]
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LYCV_BPPH2 [Bacteriophage phi-29] lysozyme (ec 3.2.1.17) (lysis protein)
(muramidase) (endolysin)(morphogenesis protein 2) [258 residues]
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LYCV_BPPZA [Bacteriophage pza] lysozyme (ec 3.2.1.17) (lysis protein)
(muramidase) (endolysin)(late protein gp15) [258 residues]
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Q9FZW0 [Bacteriophage ga-1] peptidoglycan
hydrolase [239 residues]
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Keyword search for PF01476 (LysM) at Washington St. Louis brings
up related family called TailX (PF05489) which has yet again a different
set of viral genes.
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156 members in a3, many hydrolases, some eucaryotic contamination.
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413 members in b1, including Mx8, Dp-1, SM-1, bIL285=pi2,6=pi3, BK5-T,
Che9d gp17, Bxz1, phage 2120, phiE125, HK022, N15, HK97, gifsy-2,
fels-1,
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Other names, enterotoxin, NLP/P60-family protein, transglycosylase,
N-acetylmuramoyl-L-alanine amidase, lipoprotein NlpC, invasion associated
protein p60, A13 contamination, The "invasion associated proteins"
are secreted, and bind with another domain to eucaryotic cells and tissues.
The bacteria then use the peptidoglycan binding domain to attach to said
cells or tissues.
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452 members in c3
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513 in d3; 933K, V, O, P, N, M, R; gifsy-1; lambdaSo; adh; bIL309=pi1;
lambdaSa1,2; 315.3; Cp-1; Sfi11; O1205; 7201; Sfi18; S3b; DT-1;
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e3 has 543 members
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lam_Kf1 has 658 members; lam_Kf3 had 559 members. New named phage/prophages:
BFK20
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qT99new ->lam_Kg yields 648 members in lam_Kg1. 24 new ones according
to newseqs. New named phages/prophages are: Sfi21 orf288;
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583 members in lam_Kg3.
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Check lam_Kh3 on node16; qT99new
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Searching lam_Kd3 model vs. pf00877 members and vice versa indicate that
these are essentially the same domain. lam_Kd3.mod has extended N
terminal over the pfam model, but the logo has not much conserved in it.
The sam model has several deletion vs. the pfam model. The sam model
has more viral representatives, and was able to identify the domain in
a phageD gene, whereas hmmersearch with pf00877 could not.
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Did just the N terminal level a, level b3 50 members - no obvious contamination.,
level c (started to get extraneous proteins.)
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Named phage and prophages on level b3: lambda;CP-933N; Gifsy-1; N15; HK97;
HK022; Fels-1; LambdaSo; phiE125; Barnyard;
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Additional named phages and prophages of lam_KNc1: Gifsy-2; CP-933K,P,
V; 2120;
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Do hmmscore of lam_KNb3.a2m vs lam_KNc1: to see if a threshold can be found
to exclude the noise. 1e-10 may be useful. jump b3 to lam_KNd
with 1 round threshold 1e-10 on node5. homologs.
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lam_KNd1 has 81 members. a few contaminants. Picked up Gifsy-2 and
CP-933P and phage 2120, and an unnamed prophage.
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Cut out mouse and human, then tuneup. Check on tuneup -> lam_KNd3 with
67 members. Check qT99new with 1 iter 1e-12. on node5.
-> lam_KNe
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Picked up CP-933K, CP-933V, but A13 antigen.
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NP_040599.1;
9626263; I (tail component;223); tail component
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32 members in a3. up to 34 in b1; has A13 contamination
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Up to 35 members in c1.34 in c3
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Up to 35 in d1; converge on 34 in d3.
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evaluate A13 contamination
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NP_040600.1;
9626264; J (tail:host specificity;1132); tail:host specificity
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129 members in a3. tail fiber; many titan and other eucaryotic contaminants.
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12720 members in b1
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special case.
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NP_040601.1;
9626265; lom (outer host membrane;206a); outer host membrane
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47 members; homologue of ompX; variety of phages carry members as invasion
protein
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Up to 52 in b1
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stalled at 52 in c1.
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NP_040602.1;
9626266; orf-401; Tail fiber protein
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Side tail fiber; Includes P1, P2; GMSE-1, Mu, HP1, HP2, L413-C, P27,P-EibA,
Fels-1,Gifsy1,2;
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50 members in a3. 319 in b1. collagen contaminants.
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picks up phiKZ, phage 186, I, Bxz2, CTX, Sfi19, 21, P60, MD2, BFK20,
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Try deleting contaminants, and all that match only the collagen-like seq,
(now 119 members) and then go again.
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Failed trying to go to level c
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Collate with Sfi21 family.
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NP_040603.1;
9626267; orf206b; Hypothetical protein
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Only 2 members in a3; stalled at b1
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stalled.
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NP_040604.1;
9626268; orf-314; (side) Tail fiber
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60 members in a3. Including T4 (distal subunit), Fels-1,2, Gifsy 1,2, HK97,
P1 S, P2 gpH;
-
135 members in b1. T2 gp12, HP1 orf31,HP2, FC1, AR1, SV14, TuIa,
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160 in c1; 129 in c3;
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173 in d1; 145 in d3; Bxb1 gp4; 933-H; T4 gp37 and gp12; FC1; Mu; AR1 gp12;
SV14 gp37; TuIb gp37; TuIa gp37;
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Other named genes: plasmid p15BSc/SvMr, Sc/SvP,Sc/SvM1, Sc/SvQ, Sc/SvO,
DNA inversion product; PilV; YdaB; microcystin dependent protein MdpB;
RhiB; hypothetical protein Bcv' (pinB 5' region)
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go to level e
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NP_040605.1;
9626269; orf-194; Putative fiber assembly protein
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57 members in a3, includes gifsy1,2; Fels-1,2;HK97; T4 tail fiber assembly
catalyst; P2, Mu, L413-C,APSE-1, O395, phage 186;ST64B;GMSE-1
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71 members in b1
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stalled at 69 in c1.
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NP_040606.1;
9626270; ea47
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NP_040607.1;
9626271; ea31 (296); ea31
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33 members in a3; 287 members in b1.
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HNH nuclease
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special case.
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NP_040608.1;
9626272; ea59 (525); ea59
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excinuclease ABC, subunit A; ATP binding component of ABC transporte; .phage
Hau3 resistance protein; Old Enterobacteria phage P2
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2411 members in a3.
-
study.
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NP_040609.1;
9626273; ip; integration protein
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Integrase
-
276 members in a3.
-
Should overlap the sif21 family?
-
study
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NP_040610.1;
9626274; xis (excision;72); Excisionase
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Only 5 members in a3. up to 9 in b1, including phage 21
-
stalled at 9 in c1.
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NP_597778.1;
19263393; hp393; Predicted by GeneMark; Hypothetical protein
-
3 members in a3. stalled in b1
-
stalled.
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NP_040611.1;
9626275; ea8.5 (93); ea8.5
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NP_040612.1;
9626276; ea22 (182); ea22
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17 members in a3; 29 members in b1, including VT2-Sa, HK97
gp37, HK620, H19J, HK022, epsilon 15, P22 Ead, eaa, I, phi
4795,
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47 in c1; 41 in c3;
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54 in d1; 52 in d3; ST64T Orf-109, 81, Sb31; gifsy-2; 933-V, W, O,
N, U; H19J; StxII; V;
-
took out one human seq.
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go to level e
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NP_040613.1;
9626277; orf61; Hypothetical protein
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5 members in a3.; stalled at 5 in b1
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stalled.
-
NP_040614.1;
9626278; orf63; Hypothetical protein
-
5 members in a3. stalled at 5 in b1
-
stalled.
-
NP_040615.1;
9626279; orf60a; Hypothetical protein
-
6 members in a3. stalled at 6 in b1
-
stalled.
-
NP_040616.1;
9626280; exo; exonuclease
-
24 members in a3. 116 members in b1, including pseudorabies virus alkaline
nuclease, HSV DNAse, 34.1 SPP1, A118, D3, phi 4795, VT2-Sa,
-
191 in level c1. 159 in c3
-
217 in d1; 162 d3;
-
tune d.
-
NP_040617.1;
9626281; bet (recombination;261); bet
-
10 members in a3. stalled at 10 in b1
-
stalled.
-
NP_040618.1;
9626282; gam (recombination;138); host-nuclease inhibitor protein Gam
-
13 members in a3. stalled at 13 in b1
-
stalled.
-
NP_040619.1;
9626283; kil (host-killing;54); host-killing
-
7 members in a3. stalled at 7 in b1
-
stalled.
-
NP_040620.1;
9626284; cIII (antitermination;89); antitermination
-
6 members in a3. stalled at 6 in b1
-
stalled.
-
NP_040621.1;
9626285; ssb ea10 (ssb;122); Putative single-stranded DNA binding protein
-
4 members in a3. stalled at 4 members in b1
-
stalled.
-
NP_040622.1;
9626286; ral (restriction alleviation;66); restriction alleviation
-
5 members in a3. stalled at 5 members in b1
-
stalled.
-
NP_040623.1;
9626287; orf28; Hypothetical protein
-
NP_597779.1;
19263394; SexB; Predicted by GeneMark; Superinfection exclusion protein
B
-
3 members in a3. stalled at 3 members in b1.
-
stalled.
-
NP_040625.1;
9626289; N (early gene regulator;133); early gene regulator
-
4 members in a3. up to 6 members in b1
-
stalled at 6 in c1.
-
NP_040626.1;
9626290; rexb (exclusion;144); exclusion
-
2 members in a3. stalled at 2 members in b1
-
stalled.
-
NP_040627.1;
9626291; rexa (exclusion;279); exclusion
-
NP_040628.1;
9626292; cI (repressor;237); repressor
-
167 members in a3; should overlap cI superfamily
-
2202 members in b1;
-
study.
-
NP_040629.1;
9626293; cro (antirepressor; also tof;66); antirepressor
-
4 members in a3. up to 30 in b1, but wierd members from herpes
-
stalled at 27 in c1.
-
NP_040630.1;
9626294; cII (antitermination;119); antitermination
-
17 members in a3. 19 members in cII
-
stalled at 18 in c1.
-
NP_040631.1;
9626295; O (DNA replication;299); DNA replication
-
22 members in a3. up to 26 in b1.
-
36 in c1; 30 in c3
-
33 in d1;
-
tune d.
-
NP_040632.1;
9626296; P (DNA replication;233); DNA replication
-
19 members in a3. 20 in b1
-
stalled at 20 in c1.
-
NP_040633.1;
9626297; ren (exclusion;96); ren exclusion protein
-
8 members in a3. up to 9 in b1
-
stalled at 9 in c1.
-
NP_040634.1;
9626298; Nin146 (pept unknown;146); Nin
-
19 members in a3. up to 20 in b1
-
Up to 21 in c1.; 21 in c3
-
converged to 21 in d1.
-
tune d.
-
NP_040635.1;
9626299; Nin290 (pept unknown;290); Nin protein
-
81 members in a3. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) reductase;
phage 186
-
1450 members in b1., including Rosebush, phiE125, but mostly all chromosomal
-
study.
-
NP_040636.1;
9626300; Nin57 (pept unknown;57); Nin protein
-
5 members in a3.
-
stalled at 5 members in b1 (each is lambda or P22).
-
NP_040637.1;
9626301; Nin60 (pept unknown;60); NinE
-
12 members in a3.
-
stalled at 12 members in b1. lambda, Ni12, phage 21, HK620, Lahn3,
HK97, H-19B, P22, ST64T, 933W
-
NP_040638.1;
9626302; Nin56 (pept unknown;56); NinF
-
11 members in a3.
-
stalled at 11 in b1. Similar to Nin60.
-
NP_040639.1;
9626303; Nin204 (pept unknown;204); NinG
-
23 members in a3; recombination endonuclease [Bacteriophage Nil2]
-
28 members in b1; phage 21, Nil2, 933W aka I, P22, 933K, H-19B, D3,
-
48 in c1; 43 in c3;
-
47 in d1;
-
tune d.
-
NP_040640.1;
9626304; Nin68 (pept unknown;68); NinH
-
8 members in a3
-
9 in b1. Added I, already had P22=ST64T=P22-pbi, phi 4795, 933W=VT2-Sa,
HK97
-
stalled at 9 in c1.
-
NP_040641.1;
9626305; Nin221 (pept unknown;221); Nin protein
-
502 members in a3. serine/threonine protein phosphatase; LC159;
Nil2; T5;
-
Study
-
NP_040642.1;
9626306; Q (late gene regulator;207); late gene regulator (antitermination
protein)
-
31 members in a3
-
640 members in b1; HK97, HK620, P22-pbi, HK022, Qin,
-
But then called chaparone or dna-J or heat shock protein in Fels-1 and
many chromosomal loci.
-
Study if link between Q and dnaJ.
-
NP_040643.1;
9626307; orf-64; Hypothetical protein
-
NP_040644.1;
9626308; S (cell lysis;107); cell lysis protein; holin
-
18 members in a3.
-
Up to 21 in b1; but with a mosquito protein; delete it.
-
22 in c1; c3
-
converged to 22 in d1.
-
tune d.
-
NP_040645.1;
9626309; R (cell lysis;158); cell lysis protein; endolysin
-
26 members in a3.
-
Up to 33 in c1; 30 in c3;
-
36 in d1;
-
tune d.
-
NP_040646.1;
9626310; Rz (cell lysis;153); cell lysis protein; endopeptidase
-
41 members in a3.
-
43 in b1;
-
stalled at 42 in c1..
-
NP_597780.1;
19263395; Bor; Predicted by GeneMark; Bor protein precursor
-
10 members in a3. aka DLP12, Iss, lipoprotein
-
12 in b1; stalled at 12 in c1.
-
NP_597781.1;
19263396; env; Predicted by GeneMark; putative envelope protein
-
9 members in a3.
-
stalled in b1.
-
NP_597782.1;
19263397; hp97; Predicted by GeneMark; Hypothetical protein